Histopathological and Biochemical Effect of Vitamin C and D on Phosphine-Induced Hepatotoxicity in Wistar Rats

Authors

  • Moronkeji Akinpelu Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Medical Sciences, Ondo State, Nigeria
  • Solomon Matthias Gamade Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bingham University Karu, Nasarawa State, Nigeria https://orcid.org/0000-0002-7631-8782
  • Frederick Akinbo Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, University of Benin, Edo State, Nigeria
  • Temidayo Daniel Adeniyi Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Medical Sciences, Ondo State, Nigeria
  • Adebowale Funmilola Elizebeth Department of Medical Laboratory Science, Achievers University Owo, Ondo State, Nigeria
  • Emmanuel Ifeanyi Obeagu Department of Medical Laboratory Science, Kampala International University, Uganda https://orcid.org/0000-0002-4538-0161

DOI:

https://doi.org/10.22270/ajdhs.v3i2.40

Keywords:

Metal phosphide, Carotene, Liver cell damage, Poison

Abstract

Background: Aluminum phosphide is becoming a very common agent for self-poisoning. The lack of specific antidote for phosphide poisoning has aroused the current interest of research.

Objectives: This study aimed to investigate the possible protective effect of Vitamin C and D in phosphine-induced hepatotoxicity in Wistar rats.

Methods: In this experimental study, 20 Wistar rats were randomly divided into five groups ( n = 12). Group I was apparently normal Control Group. Groups II-V were induced with 2.5 mg/kg ALP. Groups II was without further treatment while Groups III-IV received vitamin C at 100mg/kg/bw and vitamin D at 10mg/kg/bw. Group V was co-administered with vitamin C at 100mg/kg/bw and vitamin at 10mg/kg/bw. The treatments lasted for 28 days.

 Results: The results showed that Vitamin C and D (P<0.05) increased antioxidant capacity. Combined active constituents of the Vitamin C and Vitamin D raised the GPX antioxidant activities when compared to the phosphine poisoned group treated individually with Vitamin C and D. The combined activities of Vitamin C and Vitamin D showed the highest antioxidant effect as compared with the control  

Conclusion: The combined activities of Vitamin C and D supplementation improved the antioxidant defense system and histology of the liver in phosphine poisoning.

Keywords: Metal phosphide, Carotene, Liver cell damage, Poison

Author Biographies

Moronkeji Akinpelu, Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Medical Sciences, Ondo State, Nigeria

Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Medical Sciences, Ondo State, Nigeria

Solomon Matthias Gamade, Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bingham University Karu, Nasarawa State, Nigeria

Department of Medical Laboratory Science, Faculty of Allied Health Sciences, Bingham University Karu, Nasarawa State, Nigeria

Frederick Akinbo, Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, University of Benin, Edo State, Nigeria

Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, University of Benin, Edo State, Nigeria

Temidayo Daniel Adeniyi, Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Medical Sciences, Ondo State, Nigeria

Department of Medical Laboratory Science, Faculty of Allied Health Sciences, University of Medical Sciences, Ondo State, Nigeria

Adebowale Funmilola Elizebeth, Department of Medical Laboratory Science, Achievers University Owo, Ondo State, Nigeria

Department of Medical Laboratory Science, Achievers University Owo, Ondo State, Nigeria

Emmanuel Ifeanyi Obeagu, Department of Medical Laboratory Science, Kampala International University, Uganda

Department of Medical Laboratory Science, Kampala International University, Uganda

References

Garg KK. Review of aluminum phosphide poisoning. Int J Med Sci Public Health 2020; 9(7):392-400.

Eddleston M, Phillips MR. Self poisoning with pesticides. Br. Med. J. 2004; 328:42-4. https://doi.org/10.1136/bmj.328.7430.42

Hashemi-Domeneh, B., Zamani, N., Hassanian-Moghaddam, H., Rahimi, M., Shadnia, S., Erfantalab, P., & Ostadi, A. A review of aluminium phosphide poisoning and a flowchart to treat it. Arhiv Za Higijenu Rada i Toksikologiju, 2016; 67(3):183-193. https://doi.org/10.1515/aiht-2016-67-2784

Toussi, A. G. Successful treatment of aluminum phosphide; is it possible? Asia Pacific Journal of Medical Toxicology, 2017; 6(3):90-96.

Hasabo G. Aluminum Phosphide, A Serious killer in Egypt. Journal of Forensic, Toxicology and Medicolegal Analysis. 2015; 1(1):6-7.

U. Hackenberg, Chronic ingestion by rats of standard diet treated with aluminium phosphide, Toxicol. Appl. Pharmacol. 1972; 23:147-158. https://doi.org/10.1016/0041-008X(72)90214-1

Mehrpour O, Singh S: Rice tablet poisoning: a major concern in Iranian population. Hum Exp Toxicol 2010; 29:701-702. https://doi.org/10.1177/0960327109359643

Shadnia S, Soltaninejad K: Spontaneous ignition due to intentional acutealuminum phosphide poisoning. J Emerg Med. 2009; 40:179-181. https://doi.org/10.1016/j.jemermed.2009.05.028

Singh, I. Dewan, A.N. Pandey, S. Tyagi, Spectrum of unnatural fatalities in Chandigarh zone of north-west India - a 25 years autopsy study from a tertiary care hospital, J. Clin. Forensic Med. 2003; 10:145-152. https://doi.org/10.1016/S1353-1131(03)00073-7

Moghadamnia AA, Abdollahi M: An epidemiological study of poisoning in northern Islamic Republic of Iran. East Mediterr Health J. 2002; 8:88-94. https://doi.org/10.26719/2002.8.1.88

A.T. Proudfoot, Aluminium and zinc phosphide poisoning, Clin. Toxicol. 2009; 47:89-100. https://doi.org/10.1080/15563650802520675

Bajpai SR: Aluminium phosphide poisoning: management and prevention. J Indian Acad Forensic Med 2010; 32:352-354.

Mokhtari, Z., Hekmatdoost, Z., & Nourian, M. Antioxidant efficacy of vitamin D. Journal of Parathyroid Disease. 2017; 5(1):11-16. http://www.jparathyroid.com.

Wiseman H. Vitamin D is a membrane antioxidant. Ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS Lett 1993; 326:285-288. https://doi.org/10.1016/0014-5793(93)81809-E

A.J. Christophers, S. Singh, D.J. Goddard, Dangerous bodies: a case of fatal aluminium phosphide poisoning, Med. J. Aust. 2002; 176:403. https://doi.org/10.5694/j.1326-5377.2002.tb04471.x

Halvaei Z, Tehrani H, Soltaninejad K, Abdollahi M, Shadnia S. Vitamin E as a novel therapy in the treatment of acute aluminum phosphide poisoning. Turk J Med Sci 2017; 47:795-800. https://doi.org/10.3906/sag-1512-6

Bresm, M. H. A., & Habeeb, M. H. H. Effect of Vitamin D3 on some antioxidant parameters in chilled semen in Awassi Ram. Archives of Razi Institute. 2022; 78(2):721-727. https://doi.org/10.22092/ARI.2022.359482.2433 .

Hau, J., & Van Hoosier, G. Handbook of laboratory animal science, second edition: Essential principles and practices. In J. Jann Hau and Gerald L. Van Hoosier (Ed.), Handbook of Laboratory Animal Science, Second Edition: Essential Principles and Practices (Second, Vol. 2). 2002. CRC Press. https://doi.org/10.1201/9781420040913

Misra HP and Fridovich I. The role of superoxide anion I the autoxidation of epinephrine and a simple assay for superoxide dismustase. The journal of biological chemistry. 1972; 247(10):3170-3175. https://doi.org/10.1016/S0021-9258(19)45228-9

Rahman I., Kode A., Biswas S. Assay for quantitative determination of glutathione and glutathione disulfide levels using enzymatic recycling method. Nat Protoc. 2002; 1(6):3159-65. https://doi.org/10.1038/nprot.2006.378

N.S. Nath, I. Bhattacharya, A.G. Tuck, D.I. Schlipalius, P.R. Ebert, Mechanisms of phosphine toxicity, J. Toxicol. 2011; 2011:494168 https://doi.org/10.1155/2011/494168

Ali Akbar Moghadamnia. An update on toxicology of aluminum phosphide. DARU Journal of Pharmaceutical Sciences. 2012; 20:25 http://www.darujps.com/content/20/1/25. https://doi.org/10.1186/2008-2231-20-25

Saleki S, Ardalan FA, Javidan-Nejad A. Liver histopathology of fatal phosphine poisoning. Forensic Sci Int 2007; 166:190-3. https://doi.org/10.1016/j.forsciint.2006.05.033

Haghi Aminjan, H., Abtahi, S. R., Hazrati, E., Chamanara, M., Jalili, M., & Paknejad, B. Targeting of oxidative stress and inflammation through ROS/NF-kappa B pathway in phosphine-induced hepatotoxicity mitigation. Life Sciences. 2019; 232(6):116607. https://doi.org/10.1016/j.lfs.2019.116607

Abdollahi, M., & Mehrpour, O. Aluminum Phosphide. In Encyclopedia of Toxicology: Third Edition (Third Edit, Vol. 1). Elsevier. 2014. https://doi.org/10.1016/B978-0-12-386454-3.00467-X

Neki, N. S., Shergill, G. S., Singh, A., Kaur, A., Nizami, S., Singh, T., & Pannu, J. S. Recent advances in management of aluminium phosphide poisoning. Journal of Punjab Academy of Forensic Medicine and Toxicology. 2017; 17(1):53-56. https://doi.org/10.22192/ijcrms.2017.03.04.011

Karimani, A., Mohammadpour, A. H., Zirak, M. R., Rezaee, R., Megarbane, B., Tsatsakis, A., & Karimi, G. Antidotes for aluminum phosphide poisoning - An update. Toxicology Reports, 2018; 5(7):1053-1059. https://doi.org/10.1016/j.toxrep.2018.10.009

Wahba NS, Ghareib SA, Abdel-Ghany RH, Abdel-Aal M, Alsemeh AE. Vitamin D3 potentiates the nephroprotective effects of metformin in a rat model of metabolic syndrome: Role of AMPK/SIRT1 activation and DPP-4 inhibition. Canadian Journal of Physiology and Pharmacology. 2021; 99:685-697. https://doi.org/10.1139/cjpp-2020-0435

Tagliaferri S, Porri D, De Giuseppe R, Manuelli M, Alessio F, Cena H. The controversial role of vitamin D as an antioxidant: results from randomised controlled trials. Nutrition Research Reviews. 2019; 32:99-105. https://doi.org/10.1017/S0954422418000197

Schroten NF, Ruifrok WP, Kleijn L, et al. Short-term vitamin D3 supplementation lowers plasma renin activity in patients with stable chronic heart failure: an open-label, blinded end point, randomized prospective trial (VitD-CHF trial). American Heart Journal. 2013; 166:357-364. e352. https://doi.org/10.1016/j.ahj.2013.05.009

Mastali VP, Hoseini R, Azizi M. The effect of short-term vitamin D on the antioxidant capacity following exhaustive aerobic exercise. Afri Health Sci. 2023; 23(1):584-91. https://doi.org/10.4314/ahs.v23i1.61

Published

15.06.2023

How to Cite

Akinpelu, M. ., Gamade, S. M. ., Akinbo, F. ., Adeniyi, T. D. ., Elizebeth, A. F. ., & Obeagu, E. I. . (2023). Histopathological and Biochemical Effect of Vitamin C and D on Phosphine-Induced Hepatotoxicity in Wistar Rats. Asian Journal of Dental and Health Sciences, 3(2), 18–22. https://doi.org/10.22270/ajdhs.v3i2.40

Issue

Vol. 3 No. 2 (2023): Volume 3, Issue 2, April-June 2023

Section

Research Article

Citations

Copyright (c) 2023 Moronkeji Akinpelu, Solomon Matthias Gamade, Frederick Akinbo, Temidayo Daniel Adeniyi, Adebowale Funmilola Elizebeth, Emmanuel Ifeanyi Obeagu

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Crossref
Scopus
Google Scholar
Europe PMC

Most read articles by the same author(s)

1 2 > >>